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Glomalin and Conservation in Humboldt County The 1996 discovery of the soil glue glomalin is changing our understanding of the impact of elevated carbon dioxide, while giving important clues to forest health, watersheds, revegetation, wildfire and carbon sequestration. Here I share what I have found so others may read and draw their own conclusions, and relate it to my own experience, Humboldt County issues and stories from the news.
Monday, July 05, 2004
47. MATERIALS AND METHODS for GLOMALIN ANALYSIS
I have included this page for serious researchers looking to do their own work. None of these appears simple or easy to me but in the right hands this is a careers worth of raw data.
GLOMALIN A Glycoproteinaceous Substance Produced by Arbuscular Mycorrhizal Fungi
MATERIALS AND METHODS for ANALYSISSara Wright1 and Kristine Nichols2 1USDA-ARS Sustainable Agricultural Systems Laboratory, Beltsville, MD2Northern Great Plains Research Laboratory, Mandan, ND
INTRODUCTION: The following methods may be used to examine glomalin, an arbuscular mycorrhizal fungal protein, which is ubiquitous in the soil and has been found coating fungal hyphae and soil aggregates. Because of its importance in forming water-stable aggregates and in soil fertility, concentrations of this protein are being measured in a variety of soils to compare soils of different compositions and/or tillage or disruption practices. Please refer to the listed references for further details concerning the methodology and results. For further information on glomalin, please see the Glomalin Information page (PDF) file, Glomalin-Soil's Superglue, Glomalin: Hiding Place for a Third of the World's Stored Soil Carbon, and other Glomalin Research at Mandan, ND and Univeristy of Montana.Please e-mail Kris Nichols at nicholsk@mandan.ars.usda.gov to receive updates and changes to these procedures and/or continue to monitor the SASL Homepage and the INVAM website. Also, please let Kris know in your e-mail if you wish to be included on a list where ideas, questions, and methods may continue to be exchanged. In addition, if you have any improvements or additions to the current methods, please e-mail Kris so everyone can be informed.
TABLE OF CONTENTS
Pot culturing (PDF file)
Staining for percentage AM colonization (PDF file)
Indirect immuno-fluorescence assay (PDF file)
Glomalin extraction (PDF file)Bradford total protein assay (PDF file)Production of antibody against glomalin (PDF file)
ELISA immunoreactive protein assay (PDF file)
Immuno-dot blot assay (PDF file)
Glomalin Purification-Precipation and Dialysis (PDF file)
SDS-PAGE gel (PDF file)
Chemical and Materials Inventory (PDF file)
References (PDF file)
Disclaimer (PDF file)
For further information concerning glomalin, please see the Glomalin Information page. (PDF file)
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